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MedChemExpress
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Merck KGaA
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Merck KGaA
pas staining kit Pas Staining Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/pas+stain+kit/pas+staining+kit/pm32767597-68-42-45 Average 90 stars, based on 1 article reviews
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ScyTek Inc
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Merck KGaA
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Newcomer Supply Inc
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Solarbio Inc
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Carl Roth GmbH
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Morphisto GmbH
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IHC World
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Solarbio Inc
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Image Search Results
Journal: Gut and Liver
Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
doi: 10.5009/gnl18235
Figure Lengend Snippet: Hepatic differentiation ability in two-dimensional (2D) culture. (A) Gene expression of hepatic differentiation markers (AFP, ALB, and CK18) in transfected ADSCs after 2 weeks measured by qRT-PCR. (B) Amount of cytoplasmic glycogen after the overexpression of various gene in 2D culture measured by periodic acid-Schiff (PAS) staining (PAS staining: blue, nuclei; purple, glycogen in cytoplasm; magnification: ×400). The arrows indicate cytosol. AFP, alpha fetoprotein, ALB, albumin; CK18, cytokeratin 18; ADSCs, adipose tissue-derived stem cells; qRT-PCR, real-time quantitative polymerase chain reaction; Foxa2, forkhead box protein. *p<0.05.
Article Snippet: For liver function evaluation, periodic acid-Schiff staining (
Techniques: Gene Expression, Transfection, Quantitative RT-PCR, Over Expression, Staining, Derivative Assay, Real-time Polymerase Chain Reaction
Journal: Gut and Liver
Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
doi: 10.5009/gnl18235
Figure Lengend Snippet: Comparison of hepatic differentiation ability in three-dimensional (3D) culture using a scaffold. (A) qRT-PCR expression of hepatic differentiation markers (AFP, CK18, CD26, and CX32) in Foxa2-transfected ADSCs after 2 weeks in 3D culture. (B) H&E staining, periodic acid-Schiff (PAS) and cytochrome P450 immunofluorescence in Foxa2-overexpressing ADSCs in 3D culture (a and b: H&E staining, blue - nuclei, pink - cytosol, ×400; c and d: PAS staining, blue - nuclei, purple - glycogen in cytoplasm, ×1,000; e and f: blue - nuclei, green - cytochrome 450 in cytosol, ×1,000). (C) Quantification of cytochrome P450 intensity and glucose expression. qRT-PCR, real-time quantitative polymerase chain reaction; AFP, alpha fetoprotein; CK18, cytokeratin 18; CD26, dipeptidyl peptidase 4; CX32, connexin 32; ADSCs, adipose tissue-derived stem cells; Foxa2, forkhead box protein. *p<0.05.
Article Snippet: For liver function evaluation, periodic acid-Schiff staining (
Techniques: Comparison, Quantitative RT-PCR, Expressing, Transfection, Staining, Immunofluorescence, Real-time Polymerase Chain Reaction, Derivative Assay
Journal: Gut and Liver
Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
doi: 10.5009/gnl18235
Figure Lengend Snippet: Comparison of hepatocyte differentiation in a scaffold during in vivo culture. (A) In vivo culture design of scaffold preparation and implantation into nude mice. (B) Scanning electron microscopy of ADSCs in the PLGA scaffold. (C) qRT-PCR expression of hepatic differentiation markers (AFP, CK18, CD26 and CX32) in Foxa2-transfected ADSCs after 2 weeks in nude mice. (D) H&E staining, periodic acid-Schiff (PAS) staining and immunofluorescent staining for cytochrome P450 activity after Foxa2 gene overexpression in in vivo culture (a and b: H&E staining, blue - nuclei, pink - cytosol, ×400; c and d: PAS staining, blue - nuclei, purple - glycogen in cytoplasm, ×1,000; e and f: blue - nuclei, green - cytochrome 450 in cytosol, ×1,000). (E) Quantification of cytochrome P450 intensity and glucose expression. ADSCs, adipose tissue-derived stem cells; PLGA, poly(lactic-co-glycolic acid); qRT-PCR, real-time quantitative polymerase chain reaction; AFP, alpha fetoprotein; CK18, cytokeratin 18; CD26, dipeptidyl peptidase 4; CX32, connexin 32; Foxa2, forkhead box protein. *p<0.05.
Article Snippet: For liver function evaluation, periodic acid-Schiff staining (
Techniques: Comparison, In Vivo, Electron Microscopy, Quantitative RT-PCR, Expressing, Transfection, Staining, Activity Assay, Over Expression, Derivative Assay, Real-time Polymerase Chain Reaction
Journal: Gut and Liver
Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
doi: 10.5009/gnl18235
Figure Lengend Snippet: Stem cell homing. (A) Mouse and human β2-microglobulin mRNA expression was compared between the TAA-only group and the ADSCs scaffold group. (B) Immunofluorescence stain using human anti-mitochondria antibody was performed in mouse liver (×200) and implanted scaffold (×630) (immunofluorescence: blue - nuclei, green - human mitochondria). H&E staining (blue - nuclei, pink - cytosol, ×400) and periodic acid-Schiff (PAS) staining (blue - nuclei, purple - glycogen in cytoplasm, ×630) of implanted scaffold. TAA, thioacetamide; ADSCs, adipose tissue-derived stem cells; Foxa2, forkhead box protein.
Article Snippet: For liver function evaluation, periodic acid-Schiff staining (
Techniques: Expressing, Immunofluorescence, Staining, Derivative Assay