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Image Search Results
Journal: Gastroenterology
Article Title: Kr?ppel-like Factor 5 is Important for Maintenance of Crypt Architecture and Barrier Function in Mouse Intestine
doi: 10.1053/j.gastro.2011.06.086
Figure Lengend Snippet: Loss of a restricted differentiation zone and changes in epithelial cell lineage with deletion of Klf5. Paraffin-embedded colon sections from adult control and Klf5 mutant mice were stained with various epithelial markers to identify changes in differentiation. (A) Immunohistochemical staining with the colonic differentiation marker, carbonic anhydrase-I. (B) Quantitative real time polymerase chain reaction (qRT-PCR) to measure relative mRNA expression of Cdx1 and Cdx2 in colon tissues from control and mutant mice (*P < 0.001, **P = 0.004, n=3). (C) Alcian blue/Periodic acid-Schiff (PAS) staining to identify goblet cells and immunohistochemical staining of chromogranin A as a marker for enteroendocrine cells.
Article Snippet: Alcian blue/PAS staining of paraffin-embedded sections to identify goblet cells was carried out using the
Techniques: Control, Mutagenesis, Staining, Immunohistochemical staining, Marker, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing